Microbiology

Department of Microbiology

Dr Ram Manohar Lohia Institute of Medical Sciences, Lucknow


Head of the Department: Professor Jyotsna Agarwal, MD

Contact email: rmlimsmicrobiology@yahoo.com

Office Tel: 0522-6692000

History

The department started in year 2011, with offering serological services, and gradually expanded to current, state of the art Microbiological Diagnostic, Teaching and Research facilities. The department is located at sixth floor, hospital building, Dr RMLIMS. Microbiology. Department collaborates with National Vector Borne Diseases Control Programme for several infectious diseases including dengue, JE, Chikungunya, Malaria, Filaria and HIV. Department publishes an annual newsletter with hospital antibiograms which is circulated amongst all clinicians. We are recognized for MD microbiology (5 Seats) by NMC. We are actively involved in MBBS teaching.

Laboratory Facilities (Part I)

Bacteriology Lab

Bacteriology lab has facilities for isolation and identification of various pathogenic bacteria and determining their antimicrobial susceptibility by conventional as well as automated methods. It has automated continuous monitoring system of blood culture for growth of aerobic bacteria and MALDITOF is available. Automated culture method for anaerobic bacteria is also available. Rapid test for C. difficile toxin is also available.

Serology Lab

Serology lab has latex agglutination tests for ASO titres, CRP and Rheumatoid factor. It also has serological tests available for syphilis including TPHA, enteric fever(WIDAL and rapid) and leptospirosis. Procalcitonin quantitative testing by VIDAS automated analyzer is available and Anti CCP antibody test is also carried out. Chikungunya IgM ELISA and scrub typhus ELISA is also done.

Laboratory Facilities (Part II)

Mycobacteriology Lab

Mycobateriology lab has state of art facilities. Conventional and Automated Culture (MGIT 320) is done for pulmonary as well as extrapulmonary samples. First /Second line anti-mycobacterial susceptibility testing by MGIT automated method is done. LPA molecular testing for identification of MDR patients is done in pulmonary samples. CBNAAT facility is also available. The laboratory also works in collaboration with NTEP. Attached with 9-10 districts of eastern UP.

Immunology Lab

Immunology lab has HIV facility for testing. Complete TORCH profile is also done. Dengue NS1Ag and IgM Ab ELISA are available.

Laboratory Facilities (Part III)

Mycology

Routine culture and identification of fungi and automated susceptibility testing of yeasts. Latex agglutination test for Cryptococcus neoformans and mucormycosis diagnosis.

Parasitology

Identification of blood and enteric parasites. Rapid test for antigen detection of Malaria and Microfilaria and ELISA for Cysticercosis and Echinococcus.

Virology

ELISA testing for Hepatitis A, B, C, and E. JE IgM ELISA and CMV RT-PCR available. BSL-2 facility available as per NMC norms.

Molecular & Surveillance

Molecular diagnostics

Fully functional section. Offers viral load estimation for Hepatitis B and C by IVD approved Cobas Techman Real-time PCR system.

Infection Surveillance

Surveillance of ICUs, OTs and kitchen. Hand Hygiene programs for staff/doctors conducted from time to time.

ICTC

Transferred under Microbiology department. Offers CD4 testing to patients attending ART clinic.

Covid Lab

We have an ICMR approved BSL-2 lab, fully functional since April 2020, to support Covid diagnostics by performing RT-PCRs and Truenat tests, round the clock. It has the capacity of testing up to 14000 samples for RTPCR in a day.

Faculty Members

NameDesignationSection Charge
Prof. Jyotsna AgarwalProfessor and HODVirology/Molecular, HIS, BSL-3
Prof. Vineeta MittalProfessorMycobacteriology, Immunology
Prof. Manodeep SenProfessorBacteriology, Parasitology, HIS
Prof. Anupam DasProfessorBacteriology, Mycology, ICTC
Prof. Jaya GargProfessorSerology, Virology, BSL-3
Dr. Sonakshi SrivastavaAssociate ProfessorMycology co-incharge
Dr. Richa SinhaAssociate ProfessorMycobacteriology co-incharge
Dr. RimjhimAssistant ProfessorBacteriology, HIS
Dr. Shiva VermaAssistant ProfessorBacteriology co-incharge
Dr. AnuraginiAssistant ProfessorVirology co-incharge

Academic Activities

YearMonthEvent Highlights
2017NovemberCME on World Antimicrobial Awareness Week
2018MarchCME on “Fungal Sepsis” by Prof. Arunaloke Chakrabarti
2018MayHand Hygiene Day & Poster Competition
2019MarchCME on “Beyond Antibiotics” with Prof Pallab Ray
2019August1st MBBS Quiz “RAMMBO-Q”
2020MarchCME on “Unravelling the mystery of Covid-19”

Courses & Senior Residency

The department has 11 Posts of Senior residents (Post MD Microbiology) sanctioned.

  • MBBS: Started in 2017.
  • MD: 10 seats per year approved by NMC/MCI (Started 2017).
  • DMLT: Since 2013.

Training/Dissertation for Graduates/Post graduates in Microbiology: MSc and Biotechnology students undergo training and dissertation.